Two different detection systems are provided:
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- detection via Strep-Tactin® conjugates (for Strep-/Twin-Strep-tag® proteins)
- detection via monoclonal antibodies against Strep-/Twin-Strep-tag®
Read which detection system is suitable for your applications:
For direct detection of Strep-tag® fusion proteins in Western blots use:
- via chromogenic and/or chemiluminescence reaction (no secondary antibody needed!) use
- Strep-Tactin® HRP conjugate (HRP, horseradish peroxidase)
- StrepMAB-Classic HRP conjugate
- via chromogenic reaction
- Strep-Tactin® AP conjugate (AP, alkaline phosphatase)
For protein detection in ELISA use:
Strep-Tactin | Strep-Tactin®XT | StrepMAB-Classic | StrepMAB-Immo |
AP | DY-488 | DY-488 | DY-488 |
HRP | DY-549 | DY-549 | DY-549 |
unconjugated | DY-649 | DY-649 | DY-649 |
HRP | unconjugated | ||
unconjugated |
For nearly irreversible binding of StrepMAB-Immo, the Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein).
Monopoly live casino. Visualize your Strep-tag® protein Strep-Tactin®XT, StrepMAB-Classic, or StrepMAB-Immo fluorescent conjugates. They are available with:
StrepMAB-Classic |
For nearly irreversible binding of StrepMAB-Immo, the Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein).
For staining (Twin-) Strep-tagged proteins in FACS we recommend to use:
Strep-Tactin® | Strep-Tactin®XT | StrepMAB-Immo |
Strep-Tactin® PE | DY-488 | DY-488 |
Strep-Tactin® APC | DY-549 | DY-549 |
DY-649 | DY-649 |
Oregon lottery results. For nearly irreversible binding of StrepMAB-Immo, the Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein).
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